Regulatory

Part:BBa_J100236:Design

Designed by: Sarah von Euler   Group: Campbell M Lab   (2015-09-10)


rpoD IPTG induced bacterial promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

sticky ends were added to the strand which were CGAC on the left and CGCC on the right


Source

this promoter is found in phytoplasmas which are insect-transmissible and plant-pathogenic bacteria hat multiply intracellularly in plants and insects. Chihiro Miura et.al. Functional characterization of the principal sigma factor rpoD of phytoplasmas via an in vitro transcription assay

References